Article
Recycling of a single human blastomere fixed on a microscopic slide for sexing and diagnosis of specific mutations by various types of polymerase chain reaction.
Fertility and sterility - 1 Aug 1999
He Z Y, Liu H C, Mele C A, Veeck L L, Davis O, Rosenwaks Z
Abstract excerpt
OBJECTIVE: To investigate the suitability of recycling single blastomeres to assess multiple genetic variables for preimplantation genetic diagnosis. DESIGN: Prospective randomized study. SETTING: An academic medical center. PATIENT(S): Patients undergoing IVF-ET. INTERVENTION(S): Blastomeres were disaggregated from donated embryos obtained from patients. MAIN OUTCOME MEASURE(S): Polymerase chain reaction (PCR)...
Topics
- Blastomeres
- DNA Primers
- DNA, Satellite
- Embryo Transfer
- Female
- Fertilization in Vitro
- Humans
- In Vitro Techniques
- Mutation
- Polymerase Chain Reaction
- Prospective Studies
- Sex Determination Analysis
- X Chromosome
- Y Chromosome