Article
'Protected DNA Probes' capable of strong hybridization without removal of base protecting groups.
Nucleic acids research - 1 Apr 2008
Ohkubo Akihiro, Kasuya Rintaro, Sakamoto Kazushi, Miyata Kenichi, Taguchi Haruhiko, Nagasawa Hiroshi, Tsukahara Toshifumi, Watanobe Takuma, Maki Yoshiyuki, Seio Kohji, Sekine Mitsuo
Abstract excerpt
We propose a new strategy called the 'Protected DNA Probes (PDP) method' in which appropriately protected bases selectively bind to the complementary bases without the removal of their base protecting groups. Previously, we reported that 4-N-acetylcytosine oligonucleotides (ac(4)C) exhibited a higher hybridization affinity for ssDNA than the unmodified oligonucleotides. For the PDP strategy, we created a modified...
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