Evidence that the nuclease activities associated with the herpes simplex type 1 DNA polymerase are due to the 3'-5' exonuclease.

doi:10.1128/JVI.70.7.4816-4818.1996

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Article

Evidence that the nuclease activities associated with the herpes simplex type 1 DNA polymerase are due to the 3'-5' exonuclease.

Journal of virology - 1 Jul 1996

Hall J D, Orth K L, Claus-Walker D

Abstract excerpt

We investigated nuclease activities associated with the catalytic subunit of herpes simplex virus type 1 DNA polymerase. We confirm that a 3'-5' exonuclease copurifies with this enzyme. Previous reports suggested that a 5' DNase was intrinsic to the polymerase. Our preparation lacks such activity.

Topics

Animals
Cell Line
DNA-Directed DNA Polymerase
Exodeoxyribonuclease V
Exodeoxyribonucleases
Herpesvirus 1, Human
Humans
Mutation
Spodoptera
Substrate Specificity
Viral Proteins

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