Article
The DNA binding site(s) of the Escherichia coli RecA protein.
The Journal of biological chemistry - 17 May 1996
Rehrauer W M, Kowalczykowski S C
Abstract excerpt
Photochemical cross-linking has been used to identify residues in the Escherichia coli RecA protein that are proximal to and may directly mediate binding of DNA. Ultraviolet irradiation promotes specific and efficient cross-linking of the RecA protein to poly(deoxythymidylic) acid. Cross-linked peptides remaining covalently attached to the polynucleotide following proteolytic digestion with trypsin correspond to...
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