Article
Functional characterization of residues in the P-loop motif of the RecA protein ATP binding site.
Journal of molecular biology - 18 Mar 1994
Konola J T, Logan K M, Knight K L
Abstract excerpt
We have introduced a large number of single amino acid substitutions at six positions that lie within the P-loop motif of the ATP binding site in the Escherichia coli RecA protein. The activity of each recA mutant was determined using genetic assays which assess the catalytic proficiency of the R...
Topics
- Adenosine Triphosphate
- Amino Acid Sequence
- Binding Sites
- Codon
- Conserved Sequence
- Escherichia coli
- Hydrolysis
- Models, Molecular
- Molecular Sequence Data
- Mutagenesis, Insertional
- Phenotype
- Protein Conformation
- Rec A Recombinases
- Recombination, Genetic
- Structure-Activity Relationship