Article
The C terminus of the Escherichia coli RecA protein modulates the DNA binding competition with single-stranded DNA-binding protein.
The Journal of biological chemistry - 2 May 2003
Eggler Aimee L, Lusetti Shelley L, Cox Michael M
Abstract excerpt
The nucleation step of Escherichia coli RecA filament formation on single-stranded DNA (ssDNA) is strongly inhibited by prebound E. coli ssDNA-binding protein (SSB). The capacity of RecA protein to displace SSB is dramatically enhanced in RecA proteins with C-terminal deletions. The displacement of SSB by RecA protein is progressively improved when 6, 13, and 17 C-terminal amino acids are removed from the RecA...
Read the complete abstract on PubMedTopics
- Adenosine Triphosphate
- Binding, Competitive
- DNA
- DNA, Single-Stranded
- DNA-Binding Proteins
- Escherichia coli Proteins