Article
Analysis of the DNA binding site of Escherichia coli RecA protein.
Advances in biophysics - 1 Jan 1995
Morimatsu K, Horii T
Abstract excerpt
To investigate the DNA binding site of RecA protein, we constructed 15 recA mutants having alterations in the regions homologous to the other ssDNA binding proteins. The in vivo analyses showed that the mutational change at Arg243, Lys248, Tyr264, or simultaneously at Lys6 and Lys19, or Lys6 and Lys23 caused severe defects in the recA functions, while other mutational changes did not. Purified RecA-K6A-K23A (Lys6...
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