Article
Characterization of RecA1332 in vivo and in vitro. A role for alpha-helix E as a liaison between the subunit-subunit interface and the DNA and ATP binding domains of RecA protein.
Genes to cells : devoted to molecular & cellular mechanisms - 1 Feb 1998
Bianco P R, Weinstock G M
Abstract excerpt
BACKGROUND: The RecA protein of Escherichia coli is essential for homologous recombination and induction of the SOS response. RecA has three cysteines located at positions 90, 116 and 129. Chemical modification of these residues abolishes ATP hydrolysis and repressor cleavage, and causes a reduct...
Topics
- Adenosine Triphosphatases
- Adenosine Triphosphate
- Binding Sites
- Cations, Monovalent
- Cysteine
- DNA Repair
- DNA, Bacterial
- DNA, Single-Stranded
- Escherichia coli
- Models, Molecular
- Mutagenesis
- Mutation
- Protein Structure, Secondary
- Rec A Recombinases
- Recombination, Genetic
- SOS Response, Genetics