Article
Evidence using a green fluorescent protein-glucocorticoid receptor chimera that the Ran/TC4 GTPase mediates an essential function independent of nuclear protein import.
The Journal of cell biology - 1 Jun 1996
Carey K L, Richards S A, Lounsbury K M, Macara I G
Abstract excerpt
The Ran/TC4 GTPase is required for the nuclear accumulation of artificial karyophiles in permeabilized cell assays. To investigate Ran function in a physiologically intact setting using mammalian cells, we examined the effects of several Ran mutants on cell growth and on the nuclear translocation...
Topics
- Amino Acid Sequence
- Animals
- Biological Transport, Active
- Cell Division
- Cell Line
- Cell Nucleus
- Cricetinae
- Escherichia coli
- GTP Phosphohydrolases
- Gene Expression
- Green Fluorescent Proteins
- Luminescent Proteins
- Microinjections
- Molecular Sequence Data
- Mutation