Article
Double labelling of subcellular structures with organelle-targeted GFP mutants in vivo.
Current biology : CB - 1 Feb 1996
Rizzuto R, Brini M, De Giorgi F, Rossi R, Heim R, Tsien R Y, Pozzan T
Abstract excerpt
BACKGROUND: The green fluorescent protein (GFP) of Aequorea victoria is emerging as a unique tool for monitoring complex phenomena such as gene expression and organelle structure and dynamics in living cells. The recent description of GFP mutants with modified spectral properties opens numerous new applications in cell biology. However, the expression and the characteristics of these GFP mutants in living...
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