Article
Evidence from disruption of the lmcpb gene array of Leishmania mexicana that cysteine proteinases are virulence factors.
Proceedings of the National Academy of Sciences of the United States of America - 11 Jun 1996
Mottram J C, Souza A E, Hutchison J E, Carter R, Frame M J, Coombs G H
Abstract excerpt
The mammalian form of the protozoan parasite Leishmania mexicana contains high activity of a cysteine proteinase (LmCPb) encoded on a tandem array of 19 genes (lmcpb). Homozygous null mutants for lmcpb have been produced by targeted gene disruption. All life-cycle stages of the mutant can be cult...
Topics
- Animals
- Base Sequence
- Cloning, Molecular
- Cysteine Endopeptidases
- DNA Primers
- Leishmania mexicana
- Mice
- Mice, Inbred BALB C
- Molecular Sequence Data
- Mutation
- Protozoan Proteins
- Virulence