Article
[Recombinant human hemoglobin with low oxygen affinity: additional effects of two mutations].
Transfusion clinique et biologique : journal de la Societe francaise de transfusion sanguine - 1 Jan 1995
Baudin V, Dumoulin A, Poyart C, Pagnier J
Abstract excerpt
The search for human Hb variants exhibiting a low oxygen affinity without requiring 2,3-diphosphoglycerate, together with a low oxygation rate, is of an increased interest in the view of producing an artificial oxygen carrier. We have synthesized the recombinant Hb beta 41Phe-->Tyr (rHb beta F41Y) which exhibits a low oxygen affinity due to the stabilization of the deoxy state of tetrameric Hb [1]. Interestingly,...
Topics
- Allosteric Regulation
- Aspartic Acid
- Hemoglobins
- Humans
- Kinetics
- Lysine
- Mutation
- Oxidation-Reduction
- Oxygen
- Phenylalanine
- Recombinant Proteins