Article
Cloning of genetic loci involved in endoprotease activity in Streptomyces lividans 66: a novel neutral protease gene with an adjacent divergent putative regulatory gene.
Canadian journal of microbiology - 1 Sept 1992
Butler M J, Davey C C, Krygsman P, Walczyk E, Malek L T
Abstract excerpt
A skimmed-milk clearing assay was used to identify, in a multicopy Streptomyces lividans 66 genomic library, DNA fragments that lead to increased expression of protease activity in S. lividans 66. Three independent loci were identified. The majority class (slpA, which represented 68 of 71 clones) produced large zones of clearing. Two other classes (designated slpB and slpC) showed smaller zones than slpA....
Topics
- Amino Acid Sequence
- Bacterial Proteins
- Base Sequence
- Cloning, Molecular
- DNA, Bacterial
- Endopeptidases
- Gene Deletion
- Genes, Bacterial
- Genes, Regulator
- Genomic Library