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Article

Active-site variants of Streptomyces griseus protease B with peptide-ligation activity.

Chemistry & biology - 1 Mar 2000

Elliott R J, Bennet A J, Braun C A, MacLeod A M, Borgford T J

Abstract excerpt

BACKGROUND: Peptide-ligating technologies facilitate a range of manipulations for the study of protein structure and function that are not possible using conventional genetic or mutagenic methods. To different extents, the currently available enzymatic and nonenzymatic methodologies are synthetically demanding, sequence-dependent and/or sensitive to denaturants. No single coupling method is universally...

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Identifiers

PMID: 10712933
DOI: 10.1016/s1074-5521(00)00086-7

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