Article
Active-site variants of Streptomyces griseus protease B with peptide-ligation activity.
Chemistry & biology - 1 Mar 2000
Elliott R J, Bennet A J, Braun C A, MacLeod A M, Borgford T J
Abstract excerpt
BACKGROUND: Peptide-ligating technologies facilitate a range of manipulations for the study of protein structure and function that are not possible using conventional genetic or mutagenic methods. To different extents, the currently available enzymatic and nonenzymatic methodologies are synthetically demanding, sequence-dependent and/or sensitive to denaturants. No single coupling method is universally...
Topics
- Amino Acid Sequence
- Amino Acid Substitution
- Base Sequence
- Catalytic Domain
- DNA Primers
- Enzyme Stability
- Genetic Variation
- Hydrolysis
- Kinetics
- Mutagenesis, Site-Directed
- Oligopeptides