Article
Isolation of anti-CD22 Fv with high affinity by Fv display on human cells.
Proceedings of the National Academy of Sciences of the United States of America - 20 Jun 2006
Ho Mitchell, Nagata Satoshi, Pastan Ira
Abstract excerpt
In vitro antibody affinity maturation has generally been achieved by display of mouse or human antibodies on the surface of microorganisms (phage, bacteria, and yeast). However, problems with protein folding, posttranslational modification, and codon usage still limit the number of improved antibodies that can be obtained. An ideal system would select and improve antibodies in a mammalian cell environment where...
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