Article
Development of human cytochrome P450-expressing cell lines: application in mutagenicity testing of ochratoxin A.
Cancer research - 15 Jan 1996
de Groene E M, Hassing I G, Blom M J, Seinen W, Fink-Gremmels J, Horbach G J
Abstract excerpt
With the aim to assess the involvement of distinct forms of cytochrome P450 enzymes in the activation of procarcinogens, we have developed by means of retroviral infection a series of NIH/3T3 cell lines stably expressing human CYP1A1, CYP1A2, CYP2C10, CYP2D6, and CYP2E1 cDNA. The levels of cytoch...
Topics
- 3T3 Cells
- Animals
- Carcinogens
- Cytochrome P-450 Enzyme System
- DNA Mutational Analysis
- Genetic Vectors
- Humans
- Isoenzymes
- Lac Operon
- Mice
- Mutagenicity Tests
- Mutation
- Mycotoxins
- Ochratoxins