Article
Development of human cytochrome P450-expressing cell lines: application in mutagenicity testing of ochratoxin A.
Cancer research - 15 Jan 1996
de Groene E M, Hassing I G, Blom M J, Seinen W, Fink-Gremmels J, Horbach G J
Abstract excerpt
With the aim to assess the involvement of distinct forms of cytochrome P450 enzymes in the activation of procarcinogens, we have developed by means of retroviral infection a series of NIH/3T3 cell lines stably expressing human CYP1A1, CYP1A2, CYP2C10, CYP2D6, and CYP2E1 cDNA. The levels of cytochrome P450 enzyme activities were determined using specific substrates. An increase in specific catalytic activity could...
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