Article
A NIH/3T3 cell line stably expressing human cytochrome P450-3A4 used in combination with a lacZ' shuttle vector to study mutagenicity.
European journal of pharmacology - 26 May 1995
De Groene E M, Seinen W, Horbach G J
Abstract excerpt
An NIH/3T3 cell line, stably expressing human cytochrome P450-3A4 (CYP3A4) cDNA has been developed. This cell line was used in combination with a shuttle vector, containing the bacterial lacZ' gene as reporter gene, to study mutagenicity. Ethylmethanesulphonate and aflatoxin B1 were used as model...
Topics
- Aflatoxin B1
- Cell Line
- Cell Survival
- Cytochrome P-450 CYP3A
- Cytochrome P-450 Enzyme System
- DNA
- DNA, Complementary
- Ethyl Methanesulfonate
- Genetic Vectors
- Humans
- Mixed Function Oxygenases
- Mutagens
- Mutation
- Plasmids
- RNA
- Testosterone
- Transfection