Article
Mutations in the trpD gene of Corynebacterium glutamicum confer 5-methyltryptophan resistance by encoding a feedback-resistant anthranilate phosphoribosyltransferase.
Applied and environmental microbiology - 1 Dec 1995
O'Gara J P, Dunican L K
Abstract excerpt
The trpD gene from tryptophan-hyperproducing Corynebacterium glutamicum ATCC 21850 was isolated on the basis of its ability to confer resistance to 5-methyltryptophan on wild-type C. glutamicum AS019. Comparative sequence analysis of the genes from the wild-type AS019 and ATCC 21850 trpD genes revealed two amino acid substitutions at the protein level. Further analysis demonstrated that the trpD gene product from...
Topics
- Amino Acid Sequence
- Anthranilate Phosphoribosyltransferase
- Base Sequence
- Corynebacterium
- Drug Resistance
- Feedback
- Genes, Bacterial
- Molecular Sequence Data
- Mutation
- Protein Structure, Secondary
- Tryptophan