Article
Cloning of the trp gene cluster from a tryptophan-hyperproducing strain of Corynebacterium glutamicum: identification of a mutation in the trp leader sequence.
Applied and environmental microbiology - 1 Mar 1993
Heery D M, Dunican L K
Abstract excerpt
Corynebacterium glutamicum ATCC 21850 produces up to 5 g of extracellular L-tryptophan per liter in broth culture and displays resistance to several synthetic analogs of aromatic amino acids. Here we report the cloning of the tryptophan biosynthesis (trp) gene cluster of this strain on a 14.5-kb...
Topics
- Amino Acid Sequence
- Base Sequence
- Cloning, Molecular
- Codon
- Corynebacterium
- Drug Resistance, Microbial
- Escherichia coli
- Genes, Regulator
- Genetic Complementation Test
- Molecular Sequence Data
- Multigene Family
- Mutation
- Nucleic Acid Conformation
- Operon
- Promoter Regions, Genetic
- RNA, Bacterial
- Restriction Mapping
- Sequence Homology, Amino Acid