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Article

Mapping by multifragment cloning in vivo.

Proceedings of the National Academy of Sciences of the United States of America - 5 Dec 1995

Marykwas D L, Passmore S E

Abstract excerpt

An efficient method for mapping mutations is described in which hybrid genes, derived partly from mutant and partly from wild-type DNA, are obtained in vivo by homologous recombination of multiple fragments. The recombinants are formed in a strain in which their phenotypes are immediately apparent. This method was developed to identify changes that disrupt protein-protein interactions demonstrable by the...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Identifiers

PMID: 8524832
DOI: 10.1073/pnas.92.25.11701
PMCID: PMC40470

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Source receipt: pubmed, retrieved 7 Sept 2026.

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