Mapping by multifragment cloning in vivo.

doi:10.1073/pnas.92.25.11701

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Article

Mapping by multifragment cloning in vivo.

Proceedings of the National Academy of Sciences of the United States of America - 5 Dec 1995

Marykwas D L, Passmore S E

Abstract excerpt

An efficient method for mapping mutations is described in which hybrid genes, derived partly from mutant and partly from wild-type DNA, are obtained in vivo by homologous recombination of multiple fragments. The recombinants are formed in a strain in which their phenotypes are immediately apparen...

Topics

Bacterial Proteins
Base Sequence
Chromosome Mapping
Cloning, Molecular
Escherichia coli
Molecular Sequence Data
Phenotype
Point Mutation
Protein Binding
Recombination, Genetic
Saccharomyces cerevisiae
Sequence Analysis, DNA

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