Article
Mutation detection by a two-hybrid assay.
Human molecular genetics - 1 Jun 1998
Schwartz H, Alvares C P, White M B, Fields S
Abstract excerpt
Yeast-based assays have been developed to detect inactivating mutations in human genes, but these assays generally rely on the human protein having a biological function in yeast. We describe a simple method to detect mutations by virtue of their ability to abolish a protein-protein interaction i...
Topics
- Cloning, Molecular
- Escherichia coli
- Genes, p53
- Genetic Techniques
- Humans
- Mutation
- Polymerase Chain Reaction
- Saccharomyces cerevisiae