Article
Mutation detection by a two-hybrid assay.
Human molecular genetics - 1 Jun 1998
Schwartz H, Alvares C P, White M B, Fields S
Abstract excerpt
Yeast-based assays have been developed to detect inactivating mutations in human genes, but these assays generally rely on the human protein having a biological function in yeast. We describe a simple method to detect mutations by virtue of their ability to abolish a protein-protein interaction in the yeast two-hybrid assay. By the use of direct recombinational cloning in yeast of a reverse transcription-PCR...
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