Analysis of effects of tRNA:message stability on frameshift frequency at the Escherichia coli RF2 programmed frameshift site.

doi:10.1093/nar/21.8.1837

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Article

Analysis of effects of tRNA:message stability on frameshift frequency at the Escherichia coli RF2 programmed frameshift site.

Nucleic acids research - 25 Apr 1993

Curran J F

Abstract excerpt

The codon that is in-frame prior to +1 frameshifting at the E.coli prfB (RF2 gene) frameshift site is randomized to create thirty-two variants. These alleles vary 1000-fold in frameshift-dependent expression in fusions to lacZ. Frameshifting is more frequent at sites where the in-frame codon ends...

Topics

Alleles
Base Composition
Base Sequence
Cloning, Molecular
Codon
Escherichia coli
Frameshift Mutation
Molecular Sequence Data
Peptide Termination Factors
RNA, Transfer
Uridine

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