Article
Human hepatoma cell mutant defective in cell surface protein trafficking.
The Journal of biological chemistry - 7 Jul 1995
Stockert R J, Potvin B, Tao L, Stanley P, Wolkoff A W
Abstract excerpt
To isolate a mutant liver cell defective in the endocytic pathway, a selection strategy using toxic ligands for two distinct membrane receptors was devised. Ovalbumin-gelonin and asialoorosomucoid (ASOR)-gelonin were incubated with mutagenized HuH-7 cells, and a rare survivor termed trafficking mutant 1 (Trf1) was isolated. Trf1 cells were stably 3-fold more resistant than the parental HuH-7 to both toxic...
Topics
- Asialoglycoprotein Receptor
- Asialoglycoproteins
- Biological Transport
- Carcinoma, Hepatocellular
- Cell Compartmentation
- Drug Resistance
- Endocytosis
- Endosomes
- Gene Expression
- Humans
- Liver Neoplasms