Article
Decreased frequency of somatic hypermutation and impaired affinity maturation but intact germinal center formation in mice expressing antisense RNA to DNA polymerase zeta.
Journal of immunology (Baltimore, Md. : 1950) - 1 Jul 2001
Diaz M, Verkoczy L K, Flajnik M F, Klinman N R
Abstract excerpt
To examine a role of DNA polymerase zeta in somatic hypermutation, we generated transgenic mice that express antisense RNA to a portion of mouse REV3, the gene encoding this polymerase. These mice express high levels of antisense RNA, significantly reducing the levels of endogenous mouse REV3 tra...
Topics
- Animals
- Antibody Affinity
- B-Lymphocytes
- Clone Cells
- DNA-Directed DNA Polymerase
- Down-Regulation
- Germinal Center
- Immunoglobulin Heavy Chains
- Immunoglobulin Variable Region
- Immunoglobulin lambda-Chains
- Immunologic Memory