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Article

Additivity of mutant effects assessed by binomial mutagenesis.

Proceedings of the National Academy of Sciences of the United States of America - 1 May 1993

Gregoret L M, Sauer R T

Abstract excerpt

Eleven amino acid positions in the helix-turn-helix of lambda repressor have been mutagenized by using a combinatorial method in which alanine is substituted at each position with a probability of 0.5. Approximately 25% of the 2048 proteins in the resulting binomial library are active, including some variants with as many as seven alanine substitutions. The frequency of alanine substitutions in the set of active...

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Identifiers

PMID: 8483940
DOI: 10.1073/pnas.90.9.4246
PMCID: PMC46483

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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