Article
Network of epistatic interactions in an enzyme active site revealed by large-scale deep mutational scanning.
Proceedings of the National Academy of Sciences of the United States of America - 19 Mar 2024
Judge Allison, Sankaran Banumathi, Hu Liya, Palaniappan Murugesan, Birgy André, Prasad B V Venkataram, Palzkill Timothy
Abstract excerpt
Cooperative interactions between amino acids are critical for protein function. A genetic reflection of cooperativity is epistasis, which is when a change in the amino acid at one position changes the sequence requirements at another position. To assess epistasis within an enzyme active site, we utilized CTX-M β-lactamase as a model system. CTX-M hydrolyzes β-lactam antibiotics to provide antibiotic resistance,...
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