Article
Expression of truncated forms of liver microsomal P450 cytochromes 2B4 and 2E1 in Escherichia coli: influence of NH2-terminal region on localization in cytosol and membranes.
Proceedings of the National Academy of Sciences of the United States of America - 1 Apr 1993
Pernecky S J, Larson J R, Philpot R M, Coon M J
Abstract excerpt
The currently accepted model for the membrane topology of microsomal cytochrome P450 is that of a largely cytoplasmic domain bound by only one or two transmembrane segments at the NH2 terminus. However, as we have reported previously, P450 2E1 lacking the hydrophobic NH2-terminal signal peptide,...
Topics
- Amino Acid Sequence
- Animals
- Aryl Hydrocarbon Hydroxylases
- Base Sequence
- Cell Membrane
- Cloning, Molecular
- Cytochrome P-450 CYP2E1
- Cytochrome P-450 Enzyme System
- Cytosol
- DNA
- Enzyme Induction
- Escherichia coli
- Ethanol
- Genetic Variation