Article
Expression of modified human cytochrome P450 1A1 in Escherichia coli: effects of 5' substitution, stabilization, purification, spectral characterization, and catalytic properties.
Archives of biochemistry and biophysics - 1 Aug 1994
Guo Z, Gillam E M, Ohmori S, Tukey R H, Guengerich F P
Abstract excerpt
Human cytochrome P450 (P450) 1A1 is primarily an extrahepatic enzyme and is important because of its roles in the activation of polycyclic hydrocarbons and other xenobiotic chemicals. Purification of active enzyme from human tissues has not been successful. We report the expression and purificati...
Topics
- Amino Acid Sequence
- Base Sequence
- Cytochrome P-450 CYP1A2
- Cytochrome P-450 Enzyme System
- Escherichia coli
- Genetic Variation
- Genetic Vectors
- Humans
- Molecular Sequence Data
- Nucleic Acid Conformation
- Oxidoreductases
- RNA, Messenger
- Recombinant Proteins
- Sequence Analysis
- Substrate Specificity