Article
The N-terminal hydrophobic domain of P450c21 is required for membrane insertion and enzyme stability.
The Journal of biological chemistry - 15 Jul 1993
Hsu L C, Hu M C, Cheng H C, Lu J C, Chung B C
Abstract excerpt
Microsomal cytochromes P-450 are known to be integrated into smooth endoplasmic reticulum through their hydrophobic sequences located at the N termini. The length requirement of the membrane insertion signal was determined by the generation of six plasmids encoding mutant P450c21 that lacked various portions of the N-terminal hydrophobic domains. When they were transcribed and translated in vitro in the presence...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.