Article
Optimizations to achieve high-level expression of cytochrome P450 proteins using Escherichia coli expression systems.
Protein expression and purification - 1 Nov 2013
Zelasko Susan, Palaria Amrita, Das Aditi
Abstract excerpt
Recombinant expression of membrane-bound cytochrome P450s in bacterial expression systems provide a well-established system capable of producing large yields of catalytically active protein. As the biochemical knowledge regarding cytochrome P450s increases, so does the efficiency of protein expression through various modifications that do not disrupt the functional properties of the protein of interest. Changes...
Topics
- Cloning, Molecular
- Codon
- Cytochrome P-450 Enzyme System
- Escherichia coli
- Gene Expression
- Humans
- Models, Molecular
- Mutation
- Protein Conformation
- Recombinant Proteins