Article
The amino-terminal structures that determine topological orientation of cytochrome P-450 in microsomal membrane.
The EMBO journal - 1 Aug 1990
Sato T, Sakaguchi M, Mihara K, Omura T
Abstract excerpt
We previously showed that the amino-terminal region of P-450 is responsible not only for targeting to endoplasmic reticulum (ER) membrane but also for stable anchoring to the membrane. In the present study, we introduced several mutations or deletions into the signal-anchor region of the chimeric proteins in which the amino-terminal regions of two forms of cytochrome P-450 were fused to the mature portion of...
Topics
- Amino Acid Sequence
- Animals
- Cytochrome P-450 Enzyme System
- Dogs
- Endoplasmic Reticulum
- Ethylmaleimide
- Intracellular Membranes
- Microsomes
- Molecular Sequence Data
- Mutation
- Oligonucleotide Probes