Article
Membrane topology of the Bcl-2 proto-oncogenic protein demonstrated in vitro.
The Journal of biological chemistry - 25 Mar 1990
Chen-Levy Z, Cleary M L
Abstract excerpt
The Bcl-2 oncogenic protein was synthesized in vitro and shown to post-translationally integrate asymmetrically into microsomal membranes with no requirement for an amino-terminal signal sequence. Instead, a carboxyl-terminal hydrophobic domain of Bcl-2 served as an insertion sequence essential for membrane assembly since a Bcl-2 mutant lacking this domain completely lost its ability to associate with microsomal...
Topics
- Animals
- Intracellular Membranes
- Membrane Proteins
- Microsomes
- Mutation
- Protein Biosynthesis
- Protein Conformation
- Protein Processing, Post-Translational
- Protein-Tyrosine Kinases
- Proto-Oncogene Proteins
- Proto-Oncogene Proteins c-bcl-2