Article
Expression of modified human cytochrome P450 2E1 in Escherichia coli, purification, and spectral and catalytic properties.
Archives of biochemistry and biophysics - 1 Jul 1994
Gillam E M, Guo Z, Guengerich F P
Abstract excerpt
Human cytochrome P450 (P450) 2E1 is of interest because of its role in the oxidation of numerous drugs and carcinogens. The purification of the protein from human liver is difficult, and we report the development of a system for relatively high-level expression in Escherichia coli. A cDNA was prepared from liver cDNA by polymerase chain reaction methods and several variants with modified 5'-termini were...
Topics
- Base Sequence
- Catalysis
- Chlorzoxazone
- Cloning, Molecular
- Cytochrome P-450 CYP2E1
- Cytochrome P-450 Enzyme System
- Escherichia coli
- Humans
- Hydroxylation
- Molecular Sequence Data
- Mutation