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Article

Noncomplementary DNA double-strand-break rejoining in bacterial and human cells.

Nucleic acids research - 11 Mar 1993

King J S, Valcarcel E R, Rufer J T, Phillips J W, Morgan W F

Abstract excerpt

We examined the rejoining of noncomplementary restriction enzyme-produced DNA double-strand breaks in Escherichia coli and in cultured human cells. The enzymes used in this study, ClaI, BamHI and SalI, produce double-strand breaks with 5 protruding single strands. The joining of a ClaI-produced DNA end to a BamHI-produced end or to a SalI-produced end was examined at the DNA sequence level. End rejoining in...

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Identifiers

PMID: 8464692
DOI: 10.1093/nar/21.5.1055
PMCID: PMC309262

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