Article
Appearance of tumorous phenotypes in goldfish erythrophores transfected with ras, src, and myc oncogenes and spontaneous differentiation of the transformants in vitro.
The Journal of investigative dermatology - 1 Feb 1993
Matsumoto J, Akiyama T, Nemoto N, Masahito P, Ishikawa T
Abstract excerpt
When goldfish erythrophores isolated from the skin by tissue digestion and centrifugation in a Percoll density gradient were transfected in a monolayer-culture with v-Ha-ras or v-src oncogene either singly or in combination with v-myc by means of calcium phosphate-DNA co-precipitation, there appe...
Topics
- Animals
- Cell Differentiation
- Cell Transformation, Neoplastic
- Cells, Cultured
- Chromatophores
- Gene Expression
- Genes, myc
- Genes, ras
- Genes, src
- Goldfish
- Oncogenes
- Phenotype
- Skin
- Transfection