Article
Transformation of rabbit vascular smooth muscle cells by transfection with the early region of SV40 DNA.
The American journal of pathology - 1 Feb 1990
Nachtigal M, Legrand A, Nagpal M L, Nachtigal S A, Greenspan P
Abstract excerpt
Rabbit aortic smooth muscle cells (SMC) and Rb-1 cells, a continuous line of the same origin, were transformed by transfection with pSV3-neo DNA, a plasmid containing the SV40 early region linked to the neoR resistance gene. Transformed clones were selected in G418-containing medium at a rate of...
Topics
- Actins
- Animals
- Antigens, Polyomavirus Transforming
- Cell Transformation, Viral
- Chromosome Aberrations
- Cloning, Molecular
- DNA, Viral
- Genetic Vectors
- Lipids
- Muscle, Smooth, Vascular
- Phenotype
- Plasmids
- Rabbits
- Simian virus 40
- Transcription, Genetic
- Transfection