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Article

Baculovirus expression and purification of a soluble, mutant G-protein alpha subunit.

Protein expression and purification - 1 Feb 1993

Jones T L, Woodard C, Spiegel A M

Abstract excerpt

The cDNA for the alpha i1 protein that had undergone site-directed mutagenesis to change glycine-2 to alanine was ligated into a baculovirus transfer vector. A recombinant virus was obtained by transfecting Sf9 cells with both the wild-type baculovirus DNA and the transfer vector and screening for recombinant plaques. Infection with the recombinant virus led to a high level of expression of the mutated alpha i1...

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Identifiers

PMID: 8425110
DOI: 10.1006/prep.1993.1010

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