Article
Baculovirus expression and purification of a soluble, mutant G-protein alpha subunit.
Protein expression and purification - 1 Feb 1993
Jones T L, Woodard C, Spiegel A M
Abstract excerpt
The cDNA for the alpha i1 protein that had undergone site-directed mutagenesis to change glycine-2 to alanine was ligated into a baculovirus transfer vector. A recombinant virus was obtained by transfecting Sf9 cells with both the wild-type baculovirus DNA and the transfer vector and screening fo...
Topics
- Amino Acid Sequence
- Animals
- Baculoviridae
- Base Sequence
- Cells, Cultured
- Cloning, Molecular
- DNA
- Electrophoresis, Polyacrylamide Gel
- GTP-Binding Proteins
- Guanosine 5'-O-(3-Thiotriphosphate)
- Insecta
- Molecular Sequence Data
- Mutation
- Rats