Article
Purification and characterization of the G203T mutant alpha i-2 subunit of GTP-binding protein expressed in baculovirus-infected Sf9 cells.
Journal of biochemistry - 1 Sept 1995
Inoue S, Hoshino S, Kukimoto I, Ui M, Katada T
Abstract excerpt
We expressed the Gly203-->Thr (G203T) mutant of Gi2alpha, which was expected to show a dominant-negative phenotype in Gi2-mediated signal transduction, in baculovirus-inefected Sf9 cells and purified the mutant alpha subunit for its characterization. The rate of dissociation of GDP from G203T Gi2...
Topics
- Animals
- Baculoviridae
- Base Sequence
- DNA, Complementary
- GTP-Binding Proteins
- Guanosine 5'-O-(3-Thiotriphosphate)
- Guanosine Diphosphate
- Guanosine Triphosphate
- Humans
- Hydrolysis
- Kinetics
- Macromolecular Substances
- Molecular Sequence Data
- Mutation
- Protein Conformation
- Rats
- Receptors, Adrenergic, alpha-2
- Recombinant Proteins