Article
Construction and analysis of deletions in the structural gene (uvrD) for DNA helicase II of Escherichia coli.
Journal of bacteriology - 1 Apr 1991
Washburn B K, Kushner S R
Abstract excerpt
DNA helicase II, the product of the uvrD gene, has been implicated in DNA repair, replication, and recombination. Because the phenotypes of individual uvrD alleles vary significantly, we constructed deletion-insertion mutations in the uvrD gene to determine the phenotype of cells lacking DNA heli...
Topics
- Adenosine Triphosphatases
- Blotting, Southern
- Cell Survival
- Chromosome Deletion
- DNA Helicases
- DNA Repair
- DNA Topoisomerases, Type I
- Electrophoresis, Polyacrylamide Gel
- Escherichia coli
- Escherichia coli Proteins
- Genes, Bacterial
- Phenotype
- Plasmids
- Radiation Tolerance
- Recombination, Genetic
- Restriction Mapping
- Transduction, Genetic
- Ultraviolet Rays