A dominant negative allele of the Escherichia coli uvrD gene encoding DNA helicase II. A biochemical and genetic characterization.

doi:10.1006/jmbi.1994.1003

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Article

A dominant negative allele of the Escherichia coli uvrD gene encoding DNA helicase II. A biochemical and genetic characterization.

Journal of molecular biology - 14 Jan 1994

George J W, Brosh R M, Matson S W

Abstract excerpt

A site-specific lysine to methionine mutation has been engineered at the invariant Lys35 residue in the ATPase A binding site of the Escherichia coli uvrD gene encoding DNA helicase II. The mutant protein (UvrDK35M) has been purified to apparent homogeneity and characterized. The kcat for DNA-dep...

Topics

Adenosine Triphosphatases
Alleles
Base Sequence
DNA Helicases
DNA Replication
Escherichia coli
Escherichia coli Proteins
Genes, Bacterial
Genes, Dominant
Molecular Sequence Data
Mutagenesis, Site-Directed
Phenotype

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