Article
A dominant negative allele of the Escherichia coli uvrD gene encoding DNA helicase II. A biochemical and genetic characterization.
Journal of molecular biology - 14 Jan 1994
George J W, Brosh R M, Matson S W
Abstract excerpt
A site-specific lysine to methionine mutation has been engineered at the invariant Lys35 residue in the ATPase A binding site of the Escherichia coli uvrD gene encoding DNA helicase II. The mutant protein (UvrDK35M) has been purified to apparent homogeneity and characterized. The kcat for DNA-dependent ATP hydrolysis was less than 0.5% that of the wild-type enzyme with no change in the apparent Km for ATP. No...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.