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Article

Biochemical characterization of Escherichia coli temperature-sensitive dnaB mutants dnaB8, dnaB252, dnaB70, dnaB43, and dnaB454.

Journal of bacteriology - 1 Feb 1995

Saluja D, Godson G N

Abstract excerpt

By use of PCR, the dnaB genes from the classical temperature-sensitive dnaB mutants PC8 (dnaB8), RS162 (dnaB252), CR34/454 (dnaB454), HfrH165/70 (dnaB70), and CR34/43 (dnaB43) were isolated. The mutant genes were sequenced, and single amino acid changes were identified in all cases. The mutant DnaB proteins were overexpressed in BL21 (DE3) cells by using the T7 based pET-11c expression vector system. The purified...

Topics

Adenosine Triphosphatases
Bacterial Proteins
DNA Helicases
DNA Primase
DNA Replication
DNA, Single-Stranded
DnaB Helicases
Escherichia coli
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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Mutation

Protein Binding

RNA Nucleotidyltransferases

RNA, Bacterial

Source

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Identifiers

PMID: 7532169
DOI: 10.1128/jb.177.4.1104-1111.1995
PMCID: PMC176710

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-7532169-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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