Intracellular location of the autolytic N-acetylmuramyl-L-alanine amidase in Bacillus subtilis 168 and in an autolysis-deficient mutant by immunoelectron microscopy.

Abstract excerpt

Antisera against purified autolytic N-acetylmuramyl-L-alanine amidase from Bacillus subtilis 168 were prepared in rabbits. They neutralized the enzymatic action of the purified amidase acting on isolated sodium dodecyl sulfate (SDS)-treated walls from the same organism. They also inhibited the lysis of native walls, but only after the walls lysed partially. Amidase adsorbed to insoluble walls still combined with...

Topics

• Antibodies, Bacterial
• Bacillus subtilis
• Bacteriolysis
• Immune Sera
• Immunohistochemistry
• Kinetics
• Microscopy, Immunoelectron
• Mutation
• N-Acetylmuramoyl-L-alanine Amidase
• Sodium Dodecyl Sulfate