Article
The Bacillus subtilis 168 alkaline phosphatase III gene: impact of a phoAIII mutation on total alkaline phosphatase synthesis.
Journal of bacteriology - 1 Jul 1990
Bookstein C, Edwards C W, Kapp N V, Hulett F M
Abstract excerpt
The first alkaline phosphatase (APase) structural gene mutant of Bacillus subtilis 168 was constructed by using a clone identified by hybridization to a synthetic degenerative oligonucleotide. The design of the probe was based on the first 29 amino acids of the sequenced mature APase III protein,...
Topics
- Alkaline Phosphatase
- Amino Acid Sequence
- Bacillus subtilis
- Cloning, Molecular
- DNA, Recombinant
- Escherichia coli
- Genes, Bacterial
- Isoenzymes
- Kinetics
- Molecular Sequence Data
- Mutation
- Plasmids
- Sequence Homology, Nucleic Acid
- Spores, Bacterial