Analysis of the autolysins of Bacillus subtilis 168 during vegetative growth and differentiation by using renaturing polyacrylamide gel electrophoresis.

Abstract excerpt

The autolysins of Bacillus subtilis 168 were analyzed by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis with substrate-containing gels. Four bands of vegetative autolytic activity of 90, 50, 34, and 30 kDa (bands A1 to A4) were detected in SDS and LiCl extracts and in native cell walls by using B. subtilis 168 vegetative cell walls as the substrate incorporated in the gel. The four enzyme...

Topics

• Autolysis
• Bacillus subtilis
• Cell Division
• Electrophoresis, Polyacrylamide Gel
• Mutation
• N-Acetylmuramoyl-L-alanine Amidase
• Protein Denaturation
• Substrate Specificity