Segmental exchanges define 4-aminopyridine binding and the inner mouth of K+ pores.

doi:10.1016/0896-6273(93)90154-j

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Article

Segmental exchanges define 4-aminopyridine binding and the inner mouth of K+ pores.

Neuron - 1 Sept 1993

Kirsch G E, Shieh C C, Drewe J A, Vener D F, Brown A M

Abstract excerpt

4-Aminopyridine (4AP) blocks the intracellular mouth of voltage-gated K+ channels. We identified critical regions for 4AP binding with chimeric channels in which segments of a low affinity clone (Kv2.1, IC50 = 18 mM) were replaced with those of a high affinity clone (Kv3.1, IC50 = 0.1 mM). 4AP se...

Topics

4-Aminopyridine
Amino Acid Sequence
Binding, Competitive
Chimera
Intracellular Membranes
Ion Channel Gating
Molecular Sequence Data
Mutation
Potassium Channels
Quaternary Ammonium Compounds

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