Article
An E. coli RuvC mutant defective in cleavage of synthetic Holliday junctions.
Nucleic acids research - 25 Jul 1993
Sharples G J, Lloyd R G
Abstract excerpt
Escherichia coli RuvC protein is a specific endonuclease that resolves recombination intermediates into viable products. The structural features needed for RuvC activity were investigated by sequencing three ruvC mutations and relating the base pair changes identified to the activity of the mutan...
Topics
- Amino Acid Sequence
- Bacterial Proteins
- Base Sequence
- Cloning, Molecular
- Codon
- Endodeoxyribonucleases
- Escherichia coli
- Escherichia coli Proteins
- Glycine
- Molecular Sequence Data
- Mutation
- Nucleotidyltransferases
- Plasmids
- Polymerase Chain Reaction
- Rec A Recombinases
- Recombinant Proteins
- Serine
- Structure-Activity Relationship