Article
Escherichia coli RuvC protein is an endonuclease that resolves the Holliday structure.
The EMBO journal - 1 Dec 1991
Iwasaki H, Takahagi M, Shiba T, Nakata A, Shinagawa H
Abstract excerpt
Genetic evidence suggests that the Escherichia coli ruvC gene is involved in DNA repair and in the late step of RecE and RecF pathway recombination. To study the biochemical properties of RuvC protein, we overproduced and highly purified the protein. By employing model substrates, we examined the possibility that RuvC protein is an endonuclease that resolves the Holliday structure, an intermediate in genetic...
Topics
- Amino Acid Sequence
- Bacterial Proteins
- Base Sequence
- Chromatography, Gel
- DNA, Bacterial
- DNA, Superhelical
- DNA-Binding Proteins
- Electrophoresis, Polyacrylamide Gel
- Endodeoxyribonucleases
- Endonucleases
- Escherichia coli