Genetic recombination in E. coli: RuvC protein cleaves Holliday junctions at resolution hotspots in vitro.

doi:10.1016/0092-8674(94)90074-4

Back to search

Article

Genetic recombination in E. coli: RuvC protein cleaves Holliday junctions at resolution hotspots in vitro.

Cell - 2 Dec 1994

Shah R, Bennett R J, West S C

Abstract excerpt

The E. coli RuvC protein resolves Holliday junctions during genetic recombination and postreplication repair. Using recombination intermediates made by RecA protein, we have identified specific "hotspots" for RuvC resolution. Characterization of these sites reveals a common tetranucleotide sequen...

Topics

Bacterial Proteins
Base Sequence
Consensus Sequence
DNA, Bacterial
Endodeoxyribonucleases
Escherichia coli
Escherichia coli Proteins
Manganese
Molecular Sequence Data
Mutation
Nucleic Acid Conformation
Recombination, Genetic
Substrate Specificity

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.