Article
Introduction of a point mutation into the mouse genome by homologous recombination in embryonic stem cells using a replacement type vector with a selectable marker.
Nucleic acids research - 11 Jun 1993
Rubinstein M, Japón M A, Low M J
Abstract excerpt
The introduction of small mutations instead of null alleles into the mouse genome has broad applications to the study of protein structure-function relationships and the creation of animal models of human genetic diseases. To test a simple mutational strategy we designed a targeting vector for th...
Topics
- Alleles
- Amino Acid Sequence
- Animals
- Base Sequence
- Blotting, Southern
- DNA
- Embryo, Mammalian
- Exons
- Ganciclovir
- Genetic Markers
- Genetic Vectors
- Genome
- Gentamicins
- Mice
- Molecular Sequence Data
- Mutagenesis, Insertional
- Oligodeoxyribonucleotides
- Point Mutation