Article
A targeted deletion/insertion in the mouse Pcsk1 locus is associated with homozygous embryo preimplantation lethality, mutant allele preferential transmission and heterozygous female susceptibility to dietary fat.
Developmental biology - 15 Jun 2007
Mbikay Majambu, Croissandeau Gilles, Sirois Francine, Anini Younes, Mayne Janice, Seidah Nabil G, Chrétien Michel
Abstract excerpt
Proprotein convertase 1 (PC1) is a neuroendocrine proteinase involved in the proteolytic activation of precursors to hormones and neuropeptides. To determine the physiological importance of PC1, we produced a mutant mouse from embryonic stem cells in which its locus (Pcsk1) had been inactivated by homologous recombination. The inactivating mutation consisted of a 32.7-kb internal deletion and a 1.8 kb insertion...
Topics
- Alleles
- Animals
- Blastocyst
- Dietary Fats
- Female
- Gene Deletion
- Genetic Predisposition to Disease
- Heterozygote
- Homozygote
- Male